rat anti vinculin  (Developmental Studies Hybridoma Bank)

Journal: Materials Today Bio

Article Title: Innovative 3D-printed porous tantalum cage with non-window design to accelerate spinal fusion: A proof-of-concept study

doi: 10.1016/j.mtbio.2025.101576

Figure Lengend Snippet: (A) Immunostaining of BMSCs (blue: nuclei; red: vinculin) to evaluate the cell adhesion (scale bar: 200 μm); (B) Confocal laser scanning microscopy of BMSCs (red: cytoskeleton, blue: nuclear) to evaluate the cell morphology (scale bar: 100 μm); (C and D) image semi-quantitative analysis of the cell adhesion and vinculin expression; (E) Cell viability at 3, 6 and 12 h; (F) Cell proliferation at 1, 3, and 7 days. (G) Live/dead staining fluorescence images at 1 and 3 days. (H) Morphology of cells adhering to the surface of the cages determined by SEM. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: They were then incubated overnight at 4 °C with a mouse anti-vinculin monoclonal antibody (R&D Systems, USA).

Techniques: Immunostaining, Confocal Laser Scanning Microscopy, Expressing, Staining, Fluorescence